KMID : 0613820100200111691
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Journal of Life Science 2010 Volume.20 No. 11 p.1691 ~ p.1696
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Optimization of Production Yield for Neohesperidin by Response Surface Methodology
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Yang Hee-Jong
Jeong Seong-Yeop Choi Nack-Shick Ahn Keug-Hyun Park Chan-Sun Yoon Byoung-Dae Ryu Yeon-Woo Ahn Soon-Cheol Kim Min-Soo
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Abstract
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Neohesperidin is a natural new nutrition sweetener, widely existing in plants of dry citrus peel, which can be derived from extraction. Since the sweetness is 1,300-1,500 times greater than that of sugar, neohesperidin are widely used in fruit juices, wines, beverages, bakeries and pharmaceutical formulations, and are particularly suitable for consumption by diabetic patients. However, the yield of extraction from citrus peel waste is very low. In this study optimal yield conditions were determined using response surface methodology (RSM) in order to increase the neohesperidin extraction yield. The critical factors for maximum extraction yield were selected extraction pressure (x©û), extraction time (x©ü), and concentration of ethanol (x©ý). As a result, the extraction yield was improved when the extracting pressure increased. The extraction yield also increased in a time-dependent manner. When adding ethanol as an assistance solvent to the supercritical carbon dioxide, extraction yield was increased as more ethanol concentration was added. Finally, the extraction yield of neohesperidin was improved to about 162.22% compared to ethanol extraction as a conventional method.
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KEYWORD
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Neohesperidin, response surface methodology, citrus unshiu, central composite design
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